Insulin receptor studies of erythrocytes and mononuclear leucocytes in phasic insulin diabetes mellitus

This study was designed to investigate any diferences in cellular binding of insulin between phasic insulin-dependent (malnutrition-related) diabetes mellitus (PIDDM) and insulin-dependent, non-insulin-dependent, and normal controls. Isolated, washed red and white blood cells obtained after 12 - 14hr fast, were separately incubated with varying concentrations of non-radioactive insulin, and a fixed quantity of radioactively labellede insulin. After the 3hr incubation, cells were washed with buffer, and radioactivity determined on an autogamma counter. Percentage binding, receptor sites number and affinity were all determined by linear regression of the Scathard plot. Fasting plasma insulin and glucose levels were were also assayed. The results obtained showed decreased binding of insulin in red blood cells (11.3+or -1.3%) and white blood cells 2.9 + or -o.5%) in PIDDM. This was due to decreased receptor sites (red blood cells 39+ or -11; white blood cells 0.5+ or -0.11x 10 to the 4th) as well as decreased affinity (red blood cells 0.14+ or -0.03 x 10 to the 9th M-1; white blood cells 0.17 + or -0.04 x10 to the 9th M-1) when compared to the normal and diabetic (both insulin and non-insulin-dependent) controls. Phasic insulin-dependent diabetes (malnutition-related diabetes mellitus) is characterized by decreased red and white cellular binding to insiulin, in addition to decreased production of insulin

Effect of alcohol on serum lipids in diabetics

This report emanated from a three-year study conducted at the Diabetic Outpatient Clinics convened at the Port-of-Spain and Sangre-Grande Hospitals in Trinidad. The diabetic population which participated consisted of 132 men and 245 women of African descent; and 99 men and 163 women of East Indian origin. The mean age was 50 years (ñ13). The effect of alcohol on lipid fractions varied in the races. A lower incidence of ischaemic heart disease was observed among the alcohol consumers when compared to those who abstained

The effect of demographic factors on serum lipids in diabetics of Trinida & Tobago: possible association with ischaemic ischamic heart disease

This study was undertaken in order to investigate the effect of race, sex, weight, activity level and to determine how this could possibly relate to the incidence of ischaemic heart disease (IHD). Seven hundred (700) diabetics attending outpatient clinics at Sangre-Grande and Port-of-Spain hospitals were screened. There were 132 males and 249 females of African descent; 99 males and 163 females of East Indian extract, and 19 males and 38 females of other ethnic origins. The average age of the population studied was 50 years (ñ 15). The results obtained from this study indicate that diabetes mellitus is apparently more common in women that in men, particularly in those women who have borne children, and the incidence rises markedly after menopause. East Indians have higher triglyceride (TG), very low density lipoprotein cholesterol (VLDL-C) and low density lipprotein cholesterol (LDL-C), but lower high density liprotein cholesterol (HDL-c) concentrations than their counterparts of African origin. TG, LDL-C and total serum cholesterol (TC) concentrations are highest in overweight-obese patients while HDL-C concentrations are highester in the lower weight division. The domestic working class, as well as those who were in retirement, have higher TG, LDL-C than members of ther occupational status. The fractions mainly affected by a residental area are TG, HDL-C. Moreover, diabetes mellitus and its consequent impact on lipoprotein homeostasis a greater predilection to IHD, especially among members of the East Indian community

Relationship between different lipoprotein fractions and the incidence of coronary heart disease in diabetics of Trinidad & Tobago

This study was undertaken in order to investigate the relationship between the different lipoprotein fractions and the incidence of coronary heart disease (CHD) in diabetics of Trinidad and Trinidad and Tobago. Seven hundred (700) diabetics attending outpatient clinics at Sangre-Grande and Port-Grande and Port-of-Sapin hospitals were screened.There were 132 males and 249 females of African descent; 99 males and 163 females of East Indian extraction and 19 males and 38 females of other ethnic origins. The average age of the population studied was 50 years (ñ15). From this survey, it is evident that the impact of the diabetic state on lipoprotein homeostasis results in elevation of serum lipid concentrations. The elevation of low density lipoprotein cholesterol seems to be the most reliable lipid risk indicator of the lipoprotein profile, predisposing patients to a coronary event. The data also provides unequivocal evidence that diabetic patients of Trinidad and Tobago have more coronary atherosclerosis and a higher incidence of clinical CHD than does the non-diabetic population of similar age